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Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers <t>(CD29</t> and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test
Antibodies Against Cd29, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers <t>(CD29</t> and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test
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Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers <t>(CD29</t> and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test
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cd29 pe mouse miltenyi biotec  (Miltenyi Biotec)
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Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers <t>(CD29</t> and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test
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Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers <t>(CD29</t> and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test
Hamster Anti Mouse Cd29 Bd Biosciences 562219 Ab 1115404, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers <t>(CD29</t> and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test
Anti Cd29 Antibody Conjugated With Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cd29 antibody conjugated with biotin/product/Miltenyi Biotec
Average 94 stars, based on 1 article reviews
anti cd29 antibody conjugated with biotin - by Bioz Stars, 2026-02
94/100 stars
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Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers (CD29 and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test

Journal: Journal of Translational Medicine

Article Title: Hypoxic BMSCs accelerate femur fracture healing via the METTL3/SLC2A3 m6A-glycolysis axis

doi: 10.1186/s12967-025-07510-2

Figure Lengend Snippet: Hypoxia induces higher osteogenic capacity in BMSCs . ( A ) Flow cytometry assesses the expression levels of BMSCs markers (CD29 and CD44) and another cell marker (CD45). ( B ) CCK-8 assay was conducted to assess the cell viability of BMSCs after stimulation with different concentrations of CoCl₂ for 72 h. * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA. ( C ) Alizarin Red staining detects the osteogenic capacity. ( D ) Immunofluorescence assay showed the expression level of HIF1α. ( E ) ELISA analysis of ALP levels in Ctrl and Hypoxia groups. ( F ) Runx2, OCN, and Osx biomarkers were assessed using WB. *** P < 0.001, Student’s t -test

Article Snippet: BMSC identity was confirmed by flow cytometry (NovoCyte, Agilent, USA) using antibodies against CD29 (No. 130-119-165), CD44 (No. 130-107-802), and CD45 (No. 130-128-256; 1:100 diluted, Miltenyi Biotech, Germany).

Techniques: Flow Cytometry, Expressing, Marker, CCK-8 Assay, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay